Mechanisms of RNA loading into exosomes

作者：Teresa Janas, Maja M. Janas, Maja M. Janas, Karolina Sapoń, Tadeusz Janas, Tadeusz Janas · 发表于：FEBS Letters · 年份：2015 · DOI：10.1016/j.febslet.2015.04.036 · 被引用次数：395 · 研究领域：Extracellular vesicles in disease、RNA Research and Splicing、RNA Interference and Gene Delivery

Upon fusion of multivesicular bodies (MVBs) with the plasma membrane, intraluminal vesicles (ILVs) are released into the extracellular space as exosomes. Since the lipid composition of the exosomal membrane resembles that of raft microdomains, the inward budding process involves the raft-like region of the MVB limiting membrane. Although published research suggests that cellular RNAs may be selectively sorted into exosomes, the molecular mechanisms remain elusive. In this review, we suggest that there is a continuous interaction of cellular RNAs with the outer (cytoplasmic) surface of MVBs and that the selection for incorporation of these RNAs into ILVs is based on their affinity to the raft-like region in the outer layer of the MVB membrane.