DNA Hybridization Detection with Blue Luminescent Quantum Dots and Dye-Labeled Single-Stranded DNA

作者：Hui Peng, Lijuan Zhang, Tanja H. M. Kjällman, Christian Soeller, Jadranka Travaš‐Sejdić · 发表于：Journal of the American Chemical Society · 年份：2007 · DOI：10.1021/ja0685452 · 被引用次数：268 · 研究领域：Advanced biosensing and bioanalysis techniques、Quantum Dots Synthesis And Properties、Biosensors and Analytical Detection

A simple DNA sensing platform was developed based on the fluorescence resonance energy transfer (FRET) between blue-luminescent CdTe QDs and dye-labeled ssDNA. A cationic polymer acts as a “bridge” to bring the donor to acceptor together and achieves the high FRET efficiency. The hybridization event was recognized by the different FRET efficiencies due to different interaction abilities of single-stranded and double-stranded DNA with the polymer. This platform provides a homogeneous DNA assay that takes advantage of the fluorescence detection method, but with minimal DNA modification.