The Arabidopsis Abscisic Acid Response Locus ABI4 Encodes an APETALA2 Domain Protein

作者：Ruth Finkelstein, Ming Li Wang, Tim J. Lynch, Shashirekha Rao, Howard M. Goodman · 发表于：The Plant Cell · 年份：1998 · DOI：10.1105/tpc.10.6.1043 · 被引用次数：641 · 研究领域：Plant nutrient uptake and metabolism、Plant Molecular Biology Research、Plant Stress Responses and Tolerance

Arabidopsis abscisic acid (ABA)-insensitive abi4 mutants have pleiotropic defects in seed development, including decreased sensitivity to ABA inhibition of germination and altered seed-specific gene expression. This phenotype is consistent with a role for ABI4 in regulating seed responses to ABA and/or seed-specific signals. We isolated the ABI4 gene by positional cloning and confirmed its identity by complementation analysis. The predicted protein product shows homology to a plant-specific family of transcriptional regulators characterized by a conserved DNA binding domain, the APETALA 2 domain. The single mutant allele identified has a single base pair deletion, resulting in a frameshift that should disrupt the C-terminal half of the protein but leave the presumed DNA binding domain intact. Expression analyses showed that despite the seed-specific nature of the mutant phenotype, ABI4 expression is not seed specific.