Design of primers for PCR ampiification of highly variable genomes

作者：Joaquı́n Dopazo, Alfredo Rodríguez, Juan‐Carlos Sáiz, Francisco Sobrino · 发表于：Computer applications in the biosciences · 年份：1993 · DOI：10.1093/bioinformatics/9.2.123 · 被引用次数：43 · 研究领域：Animal Disease Management and Epidemiology、Viral Infections and Immunology Research、Viral Infectious Diseases and Gene Expression in Insects

A program to aid in the search of primers for specific polymerase chain reaction (PCR) amplification of highly variable genomes is presented. It involves the derivation of variability profiles to identify optimal regions for PCR amplification, taking into account stability of DNA-primer hybrids. An application of the program to foot-and-mouth disease virus diagnosis is presented.