A prokaryotic membrane anchor sequence: carboxyl terminus of bacteriophage f1 gene III protein retains it in the membrane.

作者：Jef D. Boeke, Peter Model · 发表于：Proceedings of the National Academy of Sciences · 年份：1982 · DOI：10.1073/pnas.79.17.5200 · 被引用次数：93 · 研究领域：Protein Structure and Dynamics、Bacterial Genetics and Biotechnology、RNA and protein synthesis mechanisms

Gene III protein of bacteriophage f1 is inserted into the host cell membrane where it is assembled into phage particles. A truncated form of gene III protein, encoded by a recombinant plasmid and lacking the carboxyl terminus, does not remain in the membrane but instead appears to slip through it. Fusion of a hydrophobic "membrane anchor" from another membrane protein, the gene VIII protein, to the truncated gene III protein (by manipulation of the recombinant plasmid) restores membrane anchoring. A model for the relationship of gene III protein with the Escherichia coli membrane is discussed.