Histone acetylation and globin gene switching

作者：Tim R. Hebbes, Alan W. Thorne, Alison L. Clayton, Colyn Crane‐Robinson · 发表于：Nucleic Acids Research · 年份：1992 · DOI：10.1093/nar/20.5.1017 · 被引用次数：111 · 研究领域：Genomics and Chromatin Dynamics、RNA Interference and Gene Delivery、Animal Genetics and Reproduction

An affinity-purified antibody that recognises the epitope epsilon-acetyl lysine has been used to fractionate chicken erythrocyte mononucleosomes obtained from 5 and 15 day embryos. The antibody bound chromatin was enriched in multiply acetylated forms of the core histones H3, H4 and H2B, but not in ubiquitinated H2A. The DNA of these modified nucleosomes was probed with genomic sequences from the embryonic beta rho gene (active at 5 days) and from the adult beta A gene (active at 15 days). Both genes were found to be highly enriched in the acetylated nucleosomes fractionated from both 5 day and from 15 day erythrocytes. We conclude that globin switching is not linked to a change in acetylation status of the genes and that a 'poised' gene carries histones acetylated to a similar level as a transcriptionally active gene. Core histone acetylation is not therefore a direct consequence of the transcriptional process and might operate at the level of the globin locus as a general enabling step for transcription.