The Energy Profiling of Short Interfering RNAs Is Highly Predictive of Their Activity

作者：Laura Poliseno, Monica Evangelista, Alberto Mercatanti, Laura Mariani, Lorenzo Citti, Giuseppe Rainaldi · 发表于：Oligonucleotides · 年份：2004 · DOI：10.1089/oli.2004.14.227 · 被引用次数：17 · 研究领域：RNA Interference and Gene Delivery、Advanced biosensing and bioanalysis techniques、RNA and protein synthesis mechanisms

The technology of knockdown of gene expression by synthetic short interfering RNAs (siRNAs) is currently diffused because of its relative simplicity and reliability. Despite the claims that active siRNAs can be easily identified, how to select active siRNAs with high efficiency it is not fully defined. Here, we report that energy profiling can be used in the guidelines for siRNA design.