Simple, efficientin vitrosynthesis of capped RNA useful for direct expression of cloned eukaryoti genes

作者：Roland Contreras, Hilde Cheroutre, Wim Degrave, Walter Fiers · 发表于：Nucleic Acids Research · 年份：1982 · DOI：10.1093/nar/10.20.6353 · 被引用次数：157 · 研究领域：RNA and protein synthesis mechanisms、Molecular Biology Techniques and Applications、RNA modifications and cancer

A simple and efficient method for direct in vitro synthesis of capped transcripts of cloned eukaryotic genes is described. As an example capped transcripts were made from a plasmid containing the human fibroblast interferon gene cloned under the control of a prokaryotic promoter. These transcripts were translated in vivo in Xenopus laevis oocytes and in vitro in reticulocyte and in wheat germ cell-free protein synthesizing systems.