Repair of O6-methylguanine in adapted Escherichia coli.

作者：Paul F. Schendel, Peter Robins · 发表于：Proceedings of the National Academy of Sciences · 年份：1978 · DOI：10.1073/pnas.75.12.6017 · 被引用次数：181 · 研究领域：RNA modifications and cancer、Epigenetics and DNA Methylation、DNA Repair Mechanisms

Cells exposed to sublethal concentrations of simple alkylating agents develop resistance to their mutagenic effects. This results from the induction of a system that we have called the adaptive response. During exposure to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), Escherichia coli cells induced for the adaptive response accumulate substantially less O6-methylguanine in their DNA than control cells. If O6-methylguanine does form, adapted cells possess a repair system for removing it from their DNA. The capacity of this system is limited and the system ceases to function when too much alkylation has occurred. From this point onwards O6-methylguanine starts to accumulate, and the cells begin to develop mutations at a rate directly proportional to their rate of O6-methylguanine accumulation. Our data support the idea that the O6 methylation of guanine accounts for most MNNG-induced mutagenesis.