Monodisperse Alginate Hydrogel Microbeads for Cell Encapsulation

作者：Wei-Heong Tan, Shoji Takeuchi · 发表于：Advanced Materials · 年份：2007 · DOI：10.1002/adma.200700433 · 被引用次数：598 · 研究领域：3D Printing in Biomedical Research、Innovative Microfluidic and Catalytic Techniques Innovation、Pancreatic function and diabetes

A method that combines internal gelation with T-junction droplet formation in microfluidic devices for the production of monodisperse alginate hydrogel microbeads is described. Our approach gives high uniformity, and allows better control over both size and shape of the microbeads compared to conventional external gelation methods performed in microfluidic devices (see figure). Also, we demonstrate that our approach is mild enough to encapsulate mammalian cells (Jurkat) without loss of their viability.