Flow cytometric identification of murine neutrophils and monocytes

作者：Eric Lagasse, Irving L. Weissman · 发表于：Journal of Immunological Methods · 年份：1996 · DOI：10.1016/0022-1759(96)00138-x · 被引用次数：342 · 研究领域：Immune cells in cancer、Neutrophil, Myeloperoxidase and Oxidative Mechanisms、Reproductive System and Pregnancy

Flow cytometry and cell sorting have been employed in order to identify and purify murine neutrophils and monocytes. Using a combination of antibodies, we were able to distinguish between these two subsets of myeloid cells. The method described in this paper is simple to perform and can be applied to characterize myeloid cells from blood, spleen, bone marrow and after an induced inflammation.