Role of carbohydrates in rat leukemia cell‐liver macrophage cell contacts

作者：Jutta Schlepper‐Schäfer, Natalie J. Holl, V. Kolb‐Bachofen, Eckhard A. Friedrich, H.‐A. Kolb · 发表于：Biology of the Cell · 年份：1985 · DOI：10.1111/j.1768-322x.1985.tb00344.x · 被引用次数：13 · 研究领域：Glycosylation and Glycoproteins Research、Glycogen Storage Diseases and Myoclonus、Diet, Metabolism, and Disease

The mechanism by which macrophages recognize tumor cells is still unknown. We have studied interactions between rat liver macrophages and rat L 5222 leukemia cells. These tumor cells, but not normal leukocytes or erythrocytes, adhere to freshly isolated macrophages in vitro. Binding of tumor cells by macrophages can be inhibited by N-acetyl-D-galactosamine, D-galactose and more potently by glycoproteins with terminal N-acetyl-D-galactosamine or D-galactose residues. Tumor cell adhesion is calcium-dependent. The relevant leukemia cell membrane structures which bear terminal beta-D-galactosyl or related residues have been determined as trypsin- and pronase-sensitive, and hence may presumably be glycoproteins. The tumor cell receptor on liver macrophages appears to be a lectin with the carbohydrate specificity N-acetyl-D-galactosamine greater than D-galactose greater than L-fucose.