The oyster genome reveals stress adaptation and complexity of shell formation

作者：Guofan Zhang, Guofan Zhang, Xiaodong Fang, Ximing Guo, Li Li, Ruibang Luo, Fei Xu, Pengcheng Yang, Linlin Zhang, Xiaotong Wang, Haigang Qi, 熊志强, Huayong Que, Yinlong Xie, Peter W.H. Holland, Jordi Paps, Yabing Zhu, Fucun Wu, Yuanxin Chen, Jiafeng Wang, Jie Meng, Chunfang Peng, Jie Meng, Lan Yang, Jun Liu, Bo Wen, Qihui Zhu, Zhiyong Huang, Qihui Zhu, Yue Feng, Andrew Mount, Dennis Hedgecock, Zhe Xu, Yishuai Du, Tomislav Domazet‐Lošo, Yishuai Du, Xiaoqing Sun, Shoudu Zhang, Binghang Liu, Peizhou Cheng, Xuanting Jiang, Juan Li, Dingding Fan, Tong Wang, Wenjing Fu, Tong Wang, Bo Wang, Jibiao Zhang, Zhiyu Peng, Yingxiang Li, Maoshan Chen, Jinpeng Wang, Jinpeng Wang, Maoshan Chen, Yan He, Fengji Tan, Xiaorui Song, Qiumei Zheng, Ronglian Huang, Mei Yang, Xuedi Du, Li Chen, Mei Yang, Patrick M. Gaffney, Shan Wang, Longhai Luo, Zhicai She, Ming Yao, Wen Huang, Shu Zhang, Baoyu Huang, Yong Zhang, Tao Qu, Peixiang Ni, Guoying Miao, Haiyan Wang, Junyi Wang, Qiang Wang, Christian E. W. Steinberg, Haiyan Wang, Ning Li, Lumin Qian, Guojie Zhang, Guojie Zhang, Yingrui Li, Huanming Yang, Xiao Liu, Jian Wang, Jian Wang, Ye Yin, Jun Wang, Jun Wang · 发表于：Nature · 年份：2012 · DOI：10.1038/nature11413 · 被引用次数：2310 · 研究领域：Marine Bivalve and Aquaculture Studies、Calcium Carbonate Crystallization and Inhibition、Bacteriophages and microbial interactions

The Pacific oyster Crassostrea gigas belongs to one of the most species-rich but genomically poorly explored phyla, the Mollusca. Here we report the sequencing and assembly of the oyster genome using short reads and a fosmid-pooling strategy, along with transcriptomes of development and stress response and the proteome of the shell. The oyster genome is highly polymorphic and rich in repetitive sequences, with some transposable elements still actively shaping variation. Transcriptome studies reveal an extensive set of genes responding to environmental stress. The expansion of genes coding for heat shock protein 70 and inhibitors of apoptosis is probably central to the oyster’s adaptation to sessile life in the highly stressful intertidal zone. Our analyses also show that shell formation in molluscs is more complex than currently understood and involves extensive participation of cells and their exosomes. The oyster genome sequence fills a void in our understanding of the Lophotrochozoa. The sequencing and assembly of the highly polymorphic oyster genome through a combination of short reads and fosmid pooling, complemented with extensive transcriptome analysis of development and stress response and proteome analysis of the shell, provides new insight into oyster biology and adaptation to a highly changeable environment. Oysters are keystone species in estuarine ecology and among the most important aquaculture species worldwide. The sequencing and assembly of the genome of the ...