Expression of Thymidine Phosphorylase in Human Gastric Carcinoma

作者：Yuji Takebayashi, Kazutaka Miyadera, Shin‐ichi Akiyama, Shuichi Hokita, Kazutaka Yamada, Suminori Akiba, Yuji Yamada, Tomoyuki Sumizawa, Takashi Aikou · 发表于：Japanese Journal of Cancer Research · 年份：1996 · DOI：10.1111/j.1349-7006.1996.tb00219.x · 被引用次数：101 · 研究领域：Angiogenesis and VEGF in Cancer、Cancer Treatment and Pharmacology、Glycosylation and Glycoproteins Research

The activity of thymidine phosphorylase (dThdPase) has been reported to increase in several types of malignant tumors. Experimental evidence has shown that dThdPase is identical to platelet-derived endothelial cell growth factor, and that dThdPase has angiogenic activity. We examined the expression of dThdPase to investigate whether the expression of dThdPase correlates with angiogenesis, clinicopathologic features and the prognosis of patients with human gastric carcinomas. Microvessels were assessed by immunostaining endothelial cells for factor VIII. We counted microvessels in the tumors of 158 patients whose tumors were completely removed surgically. Microvessels were counted in a x400 field in the most active areas of neovascularization. We purified a monoclonal antibody (TMA-1) against dThdPase and studied the expression of dThdPase using TMA-1 in the same serial sections as those used for the detection of factor VIII. The correlation between angiogenesis and dThdPase, and the clinicopathological significance of dThdPase, in patients with gastric carcinoma were examined. The positive expression of dThdPase was more frequent (P < 0.001) in gastric carcinomas (67/158, 43.4%) than that in normal tissues (12/158, 7.6%). The average microvessel count in dThdPase-positive gastric carcinomas was higher (P < 0.001) than that in dThdPase-negative carcinomas. The percentage of gastric carcinoma cells expressing dThdPase was significantly correlated with the microvessel count (P <...