A Sensitive H2O2 Assay Based on Dumbbell‐like PtPd‐Fe3O4 Nanoparticles

作者：Xiaolian Sun, Shaojun Guo, Chun‐Shiang Chung, Wenlei Zhu, Shouheng Sun · 发表于：Advanced Materials · 年份：2012 · DOI：10.1002/adma.201203218 · 被引用次数：216 · 研究领域：Advanced Nanomaterials in Catalysis、Electrochemical sensors and biosensors、Advanced biosensing and bioanalysis techniques

Dumbbell-like Pt(48)Pd(52)-Fe(3)O(4) nanoparticles are synthesized and functionalized with oleylamine-polyethyleneglycol to serve as an efficient catalyst for H(2)O(2) reduction and tetramethylbenzidine (TMB) oxidation in biological solutions. The Pt(48)Pd(52)-Fe(3)O(4)/TMB kit is even more active than the natural enzyme for H(2)O(2) detection with a detection limit reaching 2 μM, and is successfully used to quantitatively monitor the extracellular H(2)O(2) generated by neutrophils.