[Determination of the molecular weight of hirudin in the ultracentrifuge].

作者：H. Triebel, Peter Walsmann · 发表于：PubMed · 年份：1966 · 被引用次数：11 · 研究领域：Leech Biology and Applications

1. 1. The molecular weight has been determined of hirudin, a substance produce by the medical leech to prevent blood from coagulating. The following three independent methods were used: sedimentation velocity combined with diffusion, sedimentation equilibrium measurements in the multichannel short-column cell, and the Archibald method. From eight determinations of the molecular weight we obtained a mean value of 9060 ± 190. 2. 2. The partial specific volume of hirudin was found to be 0.741 cm3·g−1, the sedimentation coefficient at zero concentration being 0.98 S, the diffusion coefficient 10.8 F, and the molar frictional ratio 1.42. 3. 3. The hirudin preparation investigated proved to be homogeneous as to its sedimentation behavior.