Reversibility of Protein Binding of Penicillins: an In Vitro Study Employing a Rapid Diafiltration Process

作者：Michael J. Barza, Hugh Vine, Louis Weinstein · 发表于：Antimicrobial Agents and Chemotherapy · 年份：1972 · DOI：10.1128/aac.1.5.427 · 被引用次数：27 · 研究领域：Protein purification and stability、Microfluidic and Capillary Electrophoresis Applications、Protein Interaction Studies and Fluorescence Analysis

The reversibility of human serum protein binding of oxacillin, nafcillin, and cephalothin was investigated by using a diafiltration technique. While the temperature, pH, and protein concentration were kept constant, 20% of the serum volume was exchanged every 4 to 5 min. The binding of all three drugs was rapidly reversible, and the percentage of protein binding remained the same throughout the experiment.