A method for the recovery of DNA from agarose gels

作者：Henk F. Tabak, Richard Anthony Flavell · 发表于：Nucleic Acids Research · 年份：1978 · DOI：10.1093/nar/5.7.2321 · 被引用次数：264 · 研究领域：Molecular Biology Techniques and Applications、Forensic and Genetic Research、Genomics and Phylogenetic Studies

We describe a quick and versatile method for the isolation of DNA from agarose gels. The DNA is electrophoresed into a trough containing hydroxyapatite, where it is bound. The hydroxyapatite is taken out and the DNA eluted with phosphate buffer. By putting the hydroxyapatite on a small column of Sephadex G50, elution and subsequent removal of phosphate can be performed in one step. The DNA recovered can be used equally well in enzymatic incubations as DNA not purified through agarose gel electrophoresis. Several applications of this technique are described.