Polyethylene glycol-facilitated transformation of Bacteroides fragilis with plasmid DNA

作者：Charles Jeffrey Smith · 发表于：Journal of Bacteriology · 年份：1985 · DOI：10.1128/jb.164.1.466-469.1985 · 被引用次数：37 · 研究领域：Bacterial Genetics and Biotechnology、Genomics and Phylogenetic Studies、Microbial Community Ecology and Physiology

A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for the preparation of frozen competent cells is also described.