The primary structure of a plant storage protein: zein

作者：Dan E. Geraghty, Mark A. Peifer, Irwin Rubenstein, Joachim W. Messing · 发表于：Nucleic Acids Research · 年份：1981 · DOI：10.1093/nar/9.19.5163 · 被引用次数：164 · 研究领域：Phytoplasmas and Hemiptera pathogens、Entomopathogenic Microorganisms in Pest Control、Wheat and Barley Genetics and Pathology

The protein sequence of a representative of the zeins, the major storage proteins of maize, has been derived from the nucleotide sequence of a zein cDNA clone. This cDNA was sequence both by the Maxam and Gilbert and the M13-dideoxy techniques. The nucleotide sequence encompasses the non-translated 3' terminus of the mRNA, the entire coding sequence specifying both the mature zein protein and a small signal peptide, and a portion of the non-translated 5' region. The deduced amino acid composition and the amino-terminal amino acid sequence closely resemble those derived from chemical analysis of the zein protein fraction. The data presented represent the first complete amino acid sequence of a plant storage protein.