The glucose distribution in 9l rat brain multicell tumor spheroids and its effect on cell necrosis

作者：Conan K. N. Li · 发表于：Cancer · 年份：1982 · DOI：10.1002/1097-0142(19821115)50:10<2066::aid-cncr2820501017>3.0.co;2-x · 被引用次数：101 · 研究领域：3D Printing in Biomedical Research、Nanopore and Nanochannel Transport Studies、Microfluidic and Capillary Electrophoresis Applications

The glucose consumption rate of 9L rat brain tumor cells was determined as a function of concentration. Glucose uptake followed Michaelis-Menten kinetics with a Km of 0.58 mM and a Vmax of 1.6 pg/cell-min. The glucose diffusion coefficient in spheroids of 9L tumor cells was determined to be 1.5 x 10(-6) cm2/s at 37 degrees C. Using these parametric values, the glucose distribution in 9L multicell spheroids was calculated and related to the viable and necrotic zones.