Peptide sequencing identifies MSS1, a modulator of HIV Tat‐mediated transactivation, as subunit 7 of the 26 S protease

作者：Wolfgang Dubiel, Katherine Ferrell, Martin C. RECHSTEINER · 发表于：FEBS Letters · 年份：1993 · DOI：10.1016/0014-5793(93)81356-5 · 被引用次数：84 · 研究领域：HIV Research and Treatment、HIV/AIDS drug development and treatment、RNA and protein synthesis mechanisms

Subunit 7 is an integral component of the human erythrocyte 26 S protease. Peptide sequence analysis reveals that 22 amino acids from the N-terminus of subunit 7 correspond exactly to the N-terminus of MSS1, a modulator of HIV gene expression. Additional internal peptides from subunit 7 obtained by CNBr cleavage also match 100% with the deduced amino acid sequence of MSS1. Based on the fact that directly sequenced peptides from subunit 7 are identical to more than 12% of the hypothetical translation product of MSS1, and the fact that the molecular weight of subunit 7 (49 kDa) corresponds to the predicted molecular weight of MSS1 (48,633 Da), we conclude that subunit 7 is MSS1.