The mRNA coding for the secreted protease transin is expressed more abundantly in malignant than in benign tumors.

作者：L M Matrisian, G. Tim Bowden, Peter Krieg, Gerhard Fürstenberger, Jean‐Paul Briand, Pierre M. Leroy, Richard Breathnach · 发表于：Proceedings of the National Academy of Sciences · 年份：1986 · DOI：10.1073/pnas.83.24.9413 · 被引用次数：260 · 研究领域：Molecular Biology Techniques and Applications、RNA Interference and Gene Delivery、Virus-based gene therapy research

Transin RNA is a 1.9-kilobase RNA transcript induced by oncogenes in rat embryo fibroblast cell lines. We show that RNA species complementary to a cloned transin cDNA are present in mouse skin squamous cell carcinomas induced by a classical initiation-promotion protocol but not in premalignant, benign papillomas or in normal epidermis. A single application of a tumor promoting phorbol ester to normal epidermis elicits a transient increase in these RNA levels. Transin RNA encodes a secreted protease, an activity consistent with a functional role for enhanced expression of transin RNA in the progression of benign, encapsulated tumors to malignant, invasive carcinomas.