A novel mediator between activator proteins and the RNA polymerase II transcription apparatus

作者：Raymond J. Kelleher, Peter M. Flanagan, Roger D. Kornberg · 发表于：Cell · 年份：1990 · DOI：10.1016/0092-8674(90)90685-8 · 被引用次数：470 · 研究领域：Fungal and yeast genetics research、Plant Reproductive Biology、RNA and protein synthesis mechanisms

One gene activator protein may interfere with the effects of another in eukaryotic cells. We report here that a hybrid yeast-herpes gene activator protein inhibits transcriptional activation by a thymidine-rich DNA element in yeast. This example of activator interference can be faithfully reproduced in vitro. Interference is reversed by a partially purified yeast component, but not by RNA polymerase II or various polymerase II transcription factors. We conclude that the partially purified yeast component is a novel factor, and we suggest this factor mediates the transcriptional activation process.