Determination of Enantiomers in Human Serum by Direct Injection onto a β-Cyclodextrin HPLC Bonded Phase

作者：Apryll M. Stalcup, Karen L. Williams · 发表于：Journal of Liquid Chromatography · 年份：1992 · DOI：10.1080/10826079208018807 · 被引用次数：33 · 研究领域：Analytical Chemistry and Chromatography、Metabolism and Genetic Disorders、Amino Acid Enzymes and Metabolism

A method is proposed for the direct enantiomeric analysis of chiral drugs in human serum by HPLC. The method is based on a commercially available β-cyclodextrin bonded phase which incorporates diol as well as cyclodextrin moieties. The proteinaceous components of the serum, which are shown to elute with the void volume, are well separated from the enantiomeric analytes. This allows for the rapid determination of the enantiomeric composition of the drugs.