Cationized ferritin binding to anionic surfaces in normal and aminonucleoside nephrotic kidneys

作者：Peter M. Andrews · 发表于：American Journal of Anatomy · 年份：1981 · DOI：10.1002/aja.1001620202 · 被引用次数：12 · 研究领域：Renal Diseases and Glomerulopathies、Erythrocyte Function and Pathophysiology、Complement system in diseases

The distribution and effects of polycationized ferritin (PCF) bound ot anionic sites on living plasmalemmas and basal laminae of normal and puromycin aminonucleoside nephrotic (PAN) kidneys were studied using an in vitro model system. Immersion of normal glomeruli in physiological saline solutions containing low concentrations of PCF (0.01 to 0.1 mg/ml) for 10 seconds results in preferential binding of PCF to microvillous projections on the glomerular epithelium (i.e., podocytes). Exposure to higher concentrations of PCF (1.0 mg/ml) for 10 seconds results in several layers of PCF distributed evenly over the urinary aspect of epithelial podocytes. In these short treatment times, the thin slit diaphragms which span the filtration slits appear to be impermeable to PCF. Within several minutes after PCF treatment, ferritin is found within caveolae on the surface of epithelial podocytes and within numerous pinosomes and larger endocytic vesicles within these cells. Longer treatment with PCF results in the narrowing of filtration slit spaces and the formation of junctions between adjacent podocyte foot processes. Occurringg coincident with these structural changes is a gradual accumulation of PCF in regular patches along the lamina rara externa (LRE) of the glomerular basement membrane. Loss of foot processes and accumulation of PCF in the LRE are prevented by treatment with either cytochalasin B (25 micro g/ml), D (2 micro g/ml) or incubation at low temperatures (0-4 degrees C). Wh...