RELATIONSHIP OF GERMINAL CENTERS IN LYMPHOID TISSUE TO IMMUNOLOGICAL MEMORY

作者：JAMES D. WAKEFIELD, G. J. Thorbecke · 发表于：The Journal of Experimental Medicine · 年份：1968 · DOI：10.1084/jem.128.1.171 · 被引用次数：36 · 研究领域：Xenotransplantation and immune response、Immunotoxicology and immune responses、Immune Response and Inflammation

White-pulp cells and whole spleen from donor mice immunized with sheep erythrocytes were transferred intravenously to heavily irradiated mice. The numbers of plaque-forming cells and the amount of hemagglutinating antibody produced after reexposure to antigen were measured. When reexposure to sheep erythrocytes was delayed, a much greater response occurred in the transferred cells. Peak responsiveness was reached at 24 hr after transfer. This "lag effect" was greatly reduced by repeated injections of 5-bromodeoxyuridine into the recipient mice prior to challenge with antigen. It was therefore concluded that much of the increase in responsiveness was due to a proliferation of "primed" cells after cell transfer. The fact that a significant response was given by the transferred cells in spite of 5-bromodeoxyuridine treatment suggested that some of the primed cells were nondividing. White pulp was a much richer source of responsive cells than was whole spleen.