Complete nucleotide sequence of hepatic 5-aminolaevulinate synthase precursor

作者：Iain A. Borthwick, Gopesh Srivastava, Adrienne Day, Byron A. Pirola, Mark A. Snoswell, Brian K. May, William H. Elliott · 发表于：European Journal of Biochemistry · 年份：1985 · DOI：10.1111/j.1432-1033.1985.tb09047.x · 被引用次数：70 · 研究领域：Biochemical and Molecular Research、ATP Synthase and ATPases Research、Metalloenzymes and iron-sulfur proteins

Chick embryo liver mitochondrial matrix protein, 5-aminolaevulinate synthase, is synthesised initially as a larger cytosolic precursor. In this report we present the complete nucleotide sequence of a cDNA clone coding for the precursor together with corresponding confirmatory amino acid sequence of peptides derived from purified mature mitochondrial enzyme. The deduced amino acid sequence shows that the precursor consists of mature enzyme of 579 amino acids and an N-terminal extension of 56 amino acids. The latter presequence is highly basic in character as found with other mitochondrial preproteins.