Changes in Oleic Acid Content of Transgenic Soybeans by Antisense RNA Mediated Posttranscriptional Gene Silencing

作者：Ling Zhang, Xiangdong Yang, Yuan-Yu Zhang, Jing Yang, Guangxun Qi, Dongquan Guo, Guojie Xing, Yao Yao, Wenjing Xu, Haiyun Li, Qiyun Li, Yingshan Dong · 发表于：International Journal of Genomics · 年份：2014 · DOI：10.1155/2014/921950 · 被引用次数：57 · 研究领域：Lipid metabolism and biosynthesis、Soybean genetics and cultivation

The Delta-12 oleate desaturase gene (FAD2-1), which converts oleic acid into linoleic acid, is the key enzyme determining the fatty acid composition of seed oil. In this study, we inhibited the expression of endogenous Delta-12 oleate desaturase GmFad2-1b gene by using antisense RNA in soybean Williams 82. By employing the soybean cotyledonary-node method, a part of the cDNA of soybean GmFad2-1b 801 bp was cloned for the construction of a pCAMBIA3300 vector under the soybean seed promoter BCSP. Leaf painting, LibertyLink strip, PCR, Southern blot, qRT-PCR, and fatty acid analysis were used to detect the insertion and expression of GmFad2-1b in the transgenic soybean lines. The results indicate that the metabolically engineered plants exhibited a significant increase in oleic acid (up to 51.71%) and a reduction in palmitic acid (to <3%) in their seed oil content. No structural differences were observed between the fatty acids of the transgenic and the nontransgenic oil extracts.