A cis-acting element is necessary and sufficient for translational regulation of human ferritin expression in response to iron.

作者：Matthias W. Hentze, Tracey Ann Rouault, S. Wright Caughman, Andrew Dancis, Joe B. Harford, Richard D. Klausner · 发表于：Proceedings of the National Academy of Sciences · 年份：1987 · DOI：10.1073/pnas.84.19.6730 · 被引用次数：255 · 研究领域：RNA and protein synthesis mechanisms、RNA modifications and cancer、RNA Research and Splicing

Ferritin plays a key role in determining the intracellular fate of iron and is highly regulated by the iron status of the cell. We have identified a cis-acting element in the transcribed but nontranslated 5' leader sequence of human ferritin heavy-chain mRNA. In transiently transfected murine fibroblasts, the presence of a 157-nucleotide region of the 5' leader sequence was found to be necessary for iron-dependent regulation of ferritin biosynthesis. Further, this 5' leader region is sufficient to transfer iron-mediated translational control to the expression of a heterologous gene product, chloramphenicol acetyltransferase.