Synthesis of Retinal Gangliosides During Chick Embryonic Development

作者：Pedro Panzetta, H. J. F. Macdoni, Ranwel Caputto · 发表于：Journal of Neurochemistry · 年份：1980 · DOI：10.1111/j.1471-4159.1980.tb12494.x · 被引用次数：84 · 研究领域：Glycosylation and Glycoproteins Research、ATP Synthase and ATPases Research、Protein Kinase Regulation and GTPase Signaling

Embryonic retina cells incorporated radioactivity from D-[6-3H]glucosamine into gangliosides in vitro. The incorporation was higher in retinas from younger embryos. The pattern of labeling of individual gangliosides of the retina changed gradually from a predominant labeling of gangliosides running chromatographically as GD3 (nomenclature of Svennerholm) and GM3 in retinas from 8-day-old embryos to a predominant labeling of those running as GD1a and GT1 in retinas from 13--18-day-old embryos and newly hatched chicks. The shift in the pattern of labeling correlated with a temporary increase of about sixfold of the activity of UDP-N-acetylgalactosamine:GM3 N-acetylgalactosaminyltransferase occurring between days 8 and 14 of embryonic development and with a regular increase of the activity of the UDP-galactose:GM2 galactosyltransferase occurring from day 8 until hatching. The activities of the CMP-NeuAc:lactosylceramide-, CMP-NeuAc:GM3-, and CMP-NeuAc:GM1-sialosyltransferases in the retinas of newly hatched chicks were 40, 20, and 40% in comparison with the corresponding activities determined in retinas of the 8-day-old embryo.