Specific Inhibition of Precipitation as an Aid in Antigen Analysis with Gel Diffusion Method.

作者：Bertil Björklund · 发表于：Experimental Biology and Medicine · 年份：1952 · DOI：10.3181/00379727-79-19364 · 被引用次数：88 · 研究领域：Escherichia coli research studies、Monoclonal and Polyclonal Antibodies Research、Protein purification and stability

A modification of Ouchterlony's gel diffusion method for antigen analysis is described. It is based on the following observations: 1. By pretreatment of the diffusion medium with sufficient amounts of one component of a complex immunologic system the subsequent appearance in the medium of precipitates corresponding to this particular component could be completely inhibited. 2. The diffusion of the other components of the same system and the formation of other immunoprecipitates was not influenced. 3. By incorporation in the diffusion medium of individual components of a complex immunologic system it is thus possible, by elimination of some of the lines, to simplify the precipitation spectrum and facilitate the antigen analysis.