Dehydrogenase-modified carbon-fiber microelectrodes for the measurement of neurotransmitter dynamics. 2. Covalent modification utilizing avidin-biotin technology

作者：Paul Pantano, Werner G. Kuhr · 发表于：Analytical Chemistry · 年份：1993 · DOI：10.1021/ac00053a023 · 被引用次数：104 · 研究领域：Biotin and Related Studies、Electrochemical sensors and biosensors、Molecular Junctions and Nanostructures

Dehydrogenase enzymes are immobilized onto carbon-fiber microelectrode surfaces via avidin-biotin technology and a covalently linked hydrophilic tether. The avidin-biotin coupling strategy allows the selectivity of the electrochemical measurement to be easily changed without reoptimizing the immobilization conditions. Optimized derivatization conditions are demonstrated for dimer, tetramer, and hexamer dehydrogenases. Nonelectroactive substrates (ethanol, glucose-6-phosphate, and glutamate) are quantitated through the detection of enzyme-generated NADH by fast scan cyclic staircase voltammetry (100 V/s). The glutamate dehydrogenase-modified microelectrode possesses a 300-ms response time with a detection limit of 0.5 mM glutamate and a 1-60 mM linear concentration range.