25-Hydroxyvitamin D 3 1α-Hydroxylase and Vitamin D Synthesis

作者：Ken‐ichi Takeyama, Sachiko Kitanaka, Takashi Sato, Masato Kobori, Junn Yanagisawa, Shigeaki Kato · 发表于：Science · 年份：1997 · DOI：10.1126/science.277.5333.1827 · 被引用次数：542 · 研究领域：Vitamin D Research Studies、Estrogen and related hormone effects、Hormonal Regulation and Hypertension

Renal 25-hydroxyvitamin D3 1alpha-hydroxylase [1alpha(OH)ase] catalyzes metabolic activation of 25-hydroxyvitamin D3 into 1alpha, 25-dihydroxyvitamin D3 [1alpha,25(OH)2D3], an active form of vitamin D, and is inhibited by 1alpha,25(OH)2D3. 1alpha(OH)ase, which was cloned from the kidney of mice lacking the vitamin D receptor (VDR-/- mice), is a member of the P450 family of enzymes (P450VD1alpha). Expression of 1alpha(OH)ase was suppressed by 1alpha, 25(OH)2D3 in VDR+/+ and VDR+/- mice but not in VDR-/- mice. These results indicate that the negative feedback regulation of active vitamin D synthesis is mediated by 1alpha(OH)ase through liganded VDR.