Characterization of the human receptor for T-cell growth factor.

作者：Warren J. Leonard, J M Depper, Richard J. Robb, Thomas A. Waldmann, W C Greene · 发表于：Proceedings of the National Academy of Sciences · 年份：1983 · DOI：10.1073/pnas.80.22.6957 · 被引用次数：235 · 研究领域：Galectins and Cancer Biology、Glycosylation and Glycoproteins Research、Ubiquitin and proteasome pathways

Anti-Tac monoclonal antibody has been identified as a putative antibody against the receptor for T-cell growth factor (TCGF). We now show that: (i) TCGF blocks 85% of 3H-labeled anti-Tac binding to phytohemagglutinin-activated lymphoblasts and (ii) both anti-Tac and anti-TCGF immunoprecipitate a protein band that appears to represent TCGF crosslinked to its receptor on HUT-102B2 cells. In HUT-102B2 cells, the TCGF receptor is a Mr 50,000 glycoprotein with internal disulfide bond(s) and a pI of 5.5-6.0, and it represents approximately equal to 0.05% of total cellular de novo protein synthesis. It contains a peptide of Mr 33,000 that is processed to a mature form that includes N-linked and O-linked sugars and sialic acid.