Production and Degradation of N -Acylhomoserine Lactone Quorum Sensing Signal Molecules in Bacteria Isolated from Activated Sludge

作者：Seiji Ochiai, Tomohiro Morohoshi, Ayane Kurabeishi, Masahiro Shinozaki, Haruka Fujita, Isao Sawada, Tsukasa Ikeda · 发表于：Bioscience Biotechnology and Biochemistry · 年份：2013 · DOI：10.1271/bbb.130553 · 被引用次数：48 · 研究领域：Bacterial biofilms and quorum sensing、Oral microbiology and periodontitis research、Microbial Community Ecology and Physiology

N-Acylhomoserine lactones (AHLs) function as quorum-sensing signaling molecules in many Gram-negative bacteria. We isolated a total of 672 bacterial strains from activated sludge obtained from seven sewage treatment plants in Tochigi Prefecture, Japan, and screened for AHL-producing and degrading strains. Isolates (n = 107) stimulated AHL-mediated purple pigment production in AHL reporter strains Chromobacterium violaceum CV026 and VIR07. Based on their 16S rRNA gene sequences, most of these AHL-producing isolates were assigned to the genus Aeromonas, and they were divided into six groups. Isolates (n = 46) degraded N-decanoyl-L-homoserine lactone (C10-HSL) within 24 h. Based on their 16S rRNA gene sequences, the most dominant AHL-degrading isolates were assigned to the genus Acinetobacter and divided into six groups. Strains Ooi24, Omo91, and Uzu81, which showed higher C10-HSL-degrading activity, showed putative AHL-acylase activity.