Nucleotide Sequence Coding for the Flavoprotein Subunit of the Fumarate Reductase of Escherichia coli

作者：Stewart Thomas Cole · 发表于：European Journal of Biochemistry · 年份：1982 · DOI：10.1111/j.1432-1033.1982.tb06462.x · 被引用次数：166 · 研究领域：Bacterial Genetics and Biotechnology、Protein Structure and Dynamics、RNA and protein synthesis mechanisms

The nucleotide sequence of the frdA gene, which encodes the flavoprotein subunit of the fumarate reductase, of Escherichia coli, has been determined. A polypeptide of Mr = 66,052, containing 602 amino acid residues, is predicted. In composition the FrdA protein strongly resembles the flavoprotein subunits of two succinate dehydrogenases. Moreover, a sequence of nine consecutive residues is common to the flavoprotein subunits from fumarate reductase and the beef heart succinate dehydrogenase. This sequence contains a histidyl residue which probably services as the site for attachment of the FAD cofactor to the reductase.