Transgenesis by means of blastocyst-derived embryonic stem cell lines.

作者：Achim Gossler, Thomas Doetschman, Robert W. Korn, Edgar Serfling, Rolf Kemler · 发表于：Proceedings of the National Academy of Sciences · 年份：1986 · DOI：10.1073/pnas.83.23.9065 · 被引用次数：426 · 研究领域：Animal Genetics and Reproduction、Pluripotent Stem Cells Research、CRISPR and Genetic Engineering

This study demonstrates that blastocyst-derived embryonic stem cells (ES cells) can be used as a vehicle for transgenesis. The method is nearly as efficient as other methods, and the introduced neomycin phosphotransferase (neo) gene is stably transmitted through several generations with no apparent loss in G418 resistance. An important factor contributing to the efficiency of this process is the rigorous selection, before blastocyst injection, of genetically transformed cells for in vitro developmental pluripotency. One of the advantages of the ES cell route to transgenesis is that it provides investigators with the opportunity to screen for the desired genetic alterations before reintroducing the ES cells into the animal.