Loss of Apc+ in intestinal adenomas from Min mice.

作者：Cindy Luongo, Amy Rapaich Moser, Sarah Gledhill, William F. Dove · 发表于：PubMed · 年份：1994 · 被引用次数：372 · 研究领域：Genetic factors in colorectal cancer、RNA modifications and cancer、RNA and protein synthesis mechanisms

Allelic loss at the Apc locus in spontaneously occurring intestinal adenomas from mice heterozygous for the ApcMin nonsense mutation was analyzed using a site-specific quantitative polymerase chain reaction assay. All 97 of the intestinal adenomas analyzed showed extensive loss of the wild-type Apc (Apc+) allele. Quantitative polymerase chain reaction analysis of loci linked to Apc indicated loss of the chromosome carrying Apc+. Only one copy of the homologue carrying ApcMin remained in the intestinal adenomas. Possible reasons for the difference in the mechanism of Apc+ loss between human and Min mouse intestinal adenomas are discussed.