A Receptor Component of the Chloroplast Protein Translocation Machinery

作者：Stephan Hirsch, Eva Muckel, Frank Heemeyer, Gunnar von HEIJNE, Jürgen Soll · 发表于：Science · 年份：1994 · DOI：10.1126/science.7801125 · 被引用次数：245 · 研究领域：Photosynthetic Processes and Mechanisms、Photoreceptor and optogenetics research、Mitochondrial Function and Pathology

The chloroplast outer envelope protein OEP86 functions as a receptor in precursor protein translocation into chloroplasts. Sequence analysis suggests that the precursor of OEP86 is directed to the chloroplast outer envelope by a cleavable, negatively charged, and unusually long amino-terminal peptide. This presequence is unlike other potential targeting signals and suggests the existence of another membrane insertion pathway. Insertion of precursor OEP86 required the hydrolysis of adenosine triphosphate and the existence of surface exposed chloroplast membrane components, and it was not competed by another precursor protein destined for the internal plastid compartments.