Micro-Optical Sectioning Tomography to Obtain a High-Resolution Atlas of the Mouse Brain

作者：Anan Li, Hui Gong, Bin Zhang, Qingdi Wang, Cheng Yan, Jingpeng Wu, Qian Liu, Shaoqun Zeng, Qingming Luo · 发表于：Science · 年份：2010 · DOI：10.1126/science.1191776 · 被引用次数：545 · 研究领域：Cell Image Analysis Techniques、Advanced Fluorescence Microscopy Techniques、Single-cell and spatial transcriptomics

The neuroanatomical architecture is considered to be the basis for understanding brain function and dysfunction. However, existing imaging tools have limitations for brainwide mapping of neural circuits at a mesoscale level. We developed a micro-optical sectioning tomography (MOST) system that can provide micrometer-scale tomography of a centimeter-sized whole mouse brain. Using MOST, we obtained a three-dimensional structural data set of a Golgi-stained whole mouse brain at the neurite level. The morphology and spatial locations of neurons and traces of neurites could be clearly distinguished. We found that neighboring Purkinje cells stick to each other.