Gold Nanoparticles as Carriers for A Synthetic Streptococcus Pneumoniae Type 14 Conjugate Vaccine

作者：Dodi Safari, Marco Marradi, Fabrizio Chiodo, Huberta A. T. Dekker, Yulong Shan, Roberto Adamo, Stefan Oscarson, Ger T. Rijkers, Martina Lahmann, Johannis P. Kamerling, Soledad Penadés, Harm Snippe · 发表于：Nanomedicine · 年份：2012 · DOI：10.2217/nnm.11.151 · 被引用次数：171 · 研究领域：Immunotherapy and Immune Responses、Infant Nutrition and Health、Immune responses and vaccinations

AIMS: Coupling of capsular polysaccharides of pathogens to immunogenic protein carriers (conjugate vaccines) improves carbohydrate immune response. Our idea is to explore gold nanoclusters as carriers to prepare fully synthetic carbohydrate vaccines. MATERIALS & METHODS: Gold glyconanoparticles bearing a synthetic tetrasaccharide epitope related to the Streptococcus pneumoniae type 14 capsular polysaccharide (Pn14PS), the T-helper ovalbumin 323-339 peptide (OVA(323-339)), and D-glucose were prepared by a one-pot method. Their immunogenicity was tested in mice. Cytokine levels after spleen cell stimulation with OVA(323-339) were analyzed using a luminex-multiplex cytokine assay. The capacity of the evoked antibodies to promote the uptake of S. pneumoniae type 14 by leukocytes was assessed. RESULTS & DISCUSSION: Glyconanoparticles containing 45% of tetrasaccharide and 5% OVA(323-339) triggered specific anti-Pn14PS IgG antibodies. Cytokine levels confirmed that glyconanoparticles led to T-helper cell activation. The anti-saccharide antibodies promoted the phagocytosis of type 14 bacteria by human leukocytes, indicating the functionality of the antibodies. CONCLUSION: Gold nanoparticles have great potential as carriers for the development of a great diversity of fully synthetic carbohydrate-based vaccines.