Intestinal Microbiota of 6‐week‐old Infants Across Europe: Geographic Influence Beyond Delivery Mode, Breast‐feeding, and Antibiotics

作者：Matteo Fallani, David Young, Jane Anne Scott, Elisabeth Lissa Norin, Sergio Amarri, Rüdiger Adam, Marga Aguilera, Sheila Khanna, Ángel Gil, Christine Ann Edwards, Joël Doré, Other Members of the INFABIO Team · 发表于：Journal of Pediatric Gastroenterology and Nutrition · 年份：2010 · DOI：10.1097/mpg.0b013e3181d1b11e · 被引用次数：561 · 研究领域：Gut microbiota and health、Probiotics and Fermented Foods、Clostridium difficile and Clostridium perfringens research

OBJECTIVES: : There are many differences in diet and lifestyle across Europe that may influence the development of the infant gut microbiota. This work aimed to assess the impact of geographic area, mode of delivery, feeding method, and antibiotic treatment on the fecal microbiota of infants from 5 European countries with different lifestyle characteristics: Sweden, Scotland, Germany, Italy, and Spain. PATIENTS AND METHODS: : Fecal samples from 606 infants (age 6 weeks) recruited within the European project INFABIO were analyzed by fluorescent in situ hybridization combined with flow cytometry using a panel of 10 rRNA targeted group- and species-specific oligonucleotide probes. Information on factors potentially affecting gut microbiota composition was collected with questionnaires and associations were evaluated with multivariate analyses. RESULTS: : The Bifidobacterium genus was predominant (40% average proportion of total detectable bacteria), followed by Bacteroides (11.4%) and enterobacteria (7.5%). Northern European countries were associated with higher proportions of bifidobacteria in infant feces, whereas a more diverse microbiota with more bacteroides characterized southern countries. Bifidobacteria dominated the microbiota of breast-fed infants, whereas formula-fed babies had significantly higher proportions of Bacteroides and members of the Clostridium coccoides and Lactobacillus groups. Newborns delivered by cesarean section or from mothers treated with antibiotic...