Two Cytosolic Neutrophil Oxidase Components Absent in Autosomal Chronic Granulomatous Disease

作者：Bryan D. Volpp, William Michael Nauseef, Robert Alfred Clark · 发表于：Science · 年份：1988 · DOI：10.1126/science.2848318 · 被引用次数：411 · 研究领域：Neutrophil, Myeloperoxidase and Oxidative Mechanisms、S100 Proteins and Annexins、Cell Adhesion Molecules Research

Neutrophils kill microorganisms with oxygen radicals generated by an oxidase that uses the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) as substrate. This system requires both membrane and cytosolic components and is defective in patients with chronic granulomatous disease. A cytosolic complex capable of activating latent membrane oxidase was eluted from guanosine triphosphate-agarose and was used to raise polyclonal antiserum that recognized 47- and 67-kilodalton proteins. These proteins were restricted to the cytosol of myeloid cells. Both proteins were associated with NADPH oxidase-activating capacity when neutrophil cytosol was purified on nucleotide affinity matrices or molecular sizing columns. Neutrophils from patients with two different forms of autosomal chronic granulomatous disease lacked either the 47- or 67-kilodalton protein.