Transcriptional Repression Mediated by the WT1 Wilms Tumor Gene Product

作者：Stephen L. Madden, Donna Marie Cook, Jennifer F. Morris, Andrea Gashler, Vikas P. Sukhatme, Frank J. Rauscher · 发表于：Science · 年份：1991 · DOI：10.1126/science.1654597 · 被引用次数：490 · 研究领域：Renal and related cancers

The wt1 gene, a putative tumor suppressor gene located at the Wilms tumor (WT) locus on chromosome 11p13, encodes a zinc finger-containing protein that binds to the same DNA sequence as EGR-1, a mitogen-inducible immediate-early gene product that activates transcription. The transcriptional regulatory potential of WT1 has not been demonstrated. In transient transfection assays, the WT1 protein functioned as a repressor of transcription when bound to the EGR-1 site. The repression function was mapped to the glutamine- and proline-rich NH2-terminus of WT1; fusion of this domain to the zinc finger region of EGR-1 converted EGR-1 into a transcriptional repressor.