Structure of a gap junction gene: Rat connexin-32

作者：Todd Miller, Gerhard Dahl, Rudolf Werner · 发表于：Bioscience Reports · 年份：1988 · DOI：10.1007/bf01121644 · 被引用次数：99 · 研究领域：Connexins and lens biology、Heat shock proteins research

A genomic clone for the rat liver gap junction protein (connexin-32) was isolated and characterized by restriction enzyme mapping and sequence analysis. While the complete coding sequence is contained within one uninterrupted block, the 5'-untranslated region of the transcript contains a 6.1 kb intron. Both S1 nuclease protection and primer extension assays indicate multiple transcription start sites. Sequences homologous to cAMP response elements are found near the transcription start sites and within the 3'-end of the intron.