Highly enantioselective HPLC separations using the covalently bonded macrocyclic antibiotic, ristocetin A, chiral stationary phase

作者：K. Helen Ekborg-Ott, Youbang Liu, Daniel Wayne Armstrong · 发表于：Chirality · 年份：1998 · DOI：10.1002/(sici)1520-636x(1998)10:5<434::aid-chir10>3.0.co;2-0 · 被引用次数：160 · 研究领域：Analytical Chemistry and Chromatography、Microfluidic and Capillary Electrophoresis Applications、Chromatography in Natural Products

The macrocyclic glycopeptide, ristocetin A, was covalently bonded to a silica gel support and evaluated as a liquid chromatographic (LC) chiral stationary phase (CSP). Over 230 racemates were resolved in either the reversed-phase mode, the normal-phase mode, or the polar-organic mode. The retention behavior and selectivity of this CSP were examined in each mode. Optimization of separations on this column is discussed. The ristocetin A CSP appeared to be complimentary to other glycopeptide CSPs (i.e., vancomycin and teicoplanin). Column stability was excellent. The CSP was not irreversibly altered when going from one mobile phase to another.