The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase

作者：Jonathan B. Spencer, Neal J. Stolowich, Charles A. Roessner, A. Ian Scott · 发表于：FEBS Letters · 年份：1993 · DOI：10.1016/0014-5793(93)80438-z · 被引用次数：103 · 研究领域：Enzyme Structure and Function、Bacterial Genetics and Biotechnology、Microbial Metabolic Engineering and Bioproduction

Previously, the E. coli cysG gene product had been shown to sequentially methylate uro'gen III to produce precorrin-2, hence it was given the trivial name uro'gen III methylase. We now report that in addition to methylase activity, the CysG protein catalyses both the NAD+ dependent oxidation of precorrin-2 to sirohydrochlorin, but also the insertion of iron into this oxidized intermediate, thereby producing siroheme. Thus CysG is a multifunctional protein solely responsible for siroheme synthesis from uro'gen III in E. coli, and accordingly is renamed siroheme synthase.