Distinct Functions of SR Proteins in Alternative pre-mRNA Splicing

作者：Alan M. Zahler, Karla M. Neugebauer, William S. Lane, Mark B. Roth · 发表于：Science · 年份：1993 · DOI：10.1126/science.8385799 · 被引用次数：410 · 研究领域：RNA Research and Splicing、RNA Interference and Gene Delivery、RNA and protein synthesis mechanisms

Alternative splicing of precursor messenger RNAs (pre-mRNAs) is a common mechanism of regulating gene expression. SR proteins are a family of pre-mRNA splicing factors that are structurally related and evolutionarily conserved. Any member of the SR family can complement a splicing-deficient extract that lacks the entire family of SR proteins. Here it is demonstrated that particular SR proteins have distinct functions in alternative pre-mRNA splicing in vitro. In addition, SR proteins are differentially expressed in a variety of tissues. These results suggest a fundamental role for SR proteins in the regulation of alternative splicing.