A colorimetric method for DNA hybridization

作者：Manfred Renz, Christina Kurz · 发表于：Nucleic Acids Research · 年份：1984 · DOI：10.1093/nar/12.8.3435 · 被引用次数：292 · 研究领域：Advanced biosensing and bioanalysis techniques、Animal Genetics and Reproduction、Biotin and Related Studies

A general method has been developed which allows crosslinks to be produced between proteins and single-stranded DNA. Such single-stranded DNA protein complexes have been tested for blot hybridization using two colorimetrically detectable enzymes, namely peroxidase and alkaline phosphatase, as the protein moiety of the probe. After hybridization and incubation with a substrate solution sequences complementary to the probe can be visualized directly without the need of tedious cytochemical sandwich methods. This procedure will detect target sequences, a few kilobases long, in the 1- to 5-pg range.